本实验的目的是评价不同过氧化水平的大豆油（SO）对保育猪脂质、N、GE消化率、肠道完整性、氧化应激及生长性能的影响。日粮处理组包括10%的新鲜SO（22.5℃处理），或者10%热处理SO（45℃加热288 h，90℃加热72 h，或者180℃加热6 h），每一处理都进行15 L / min的空气注入；处理后过氧化值分别为7.6、11.5、19.1和13.4 mEq/kg，混合型值分别为1.92、6.29、149和159。
在试验1中，64头仔猪（初始体重（BW）7.1±0.9 kg）被随机分配到2个饲养舍32栏中，分别饲喂为期21 d的试验饲粮，第20天采集新鲜粪便样品，测定GE和脂质消化率。在试验2中，将56头仔猪 （BW 9.16±1.56 kg）放入单独的代谢笼中，以评估GE、脂质、N的消化率和N的保留率；检测尿液中乳果糖与甘露醇的比例，以评估体内小肠的完整性；收集尿液和血浆进行氧化应激标记物的分析；随后对猪实施安乐死以获得肝脏重量，并分析肝脏中氧化应激标记物。
试验数据显示，试验1中，与其他SO处理组相比 90℃热处理的SO组猪只的ADG（P = 0.01）和ADFI（P = 0.04）显著降低，而22.5℃、45℃和180℃热处理的各SO组猪只无差异；各组间日粮GE和脂质消化率没有差异（P > 0.10）。在试验2中，各过氧化SO组间，DE：ME比值、N消化率、N的保留或者尿液中乳果糖与甘露醇比值，血清、尿液和肝脏中硫代巴比妥酸反应性物质，以及血浆蛋白质羰基、尿液和肝脏8-OH-2dG等指标均没有显著差异（P > 0.10）；与新鲜SO组比较，90℃热处理SO组猪只血清（P ≤ 0.01）和尿液（P ≤ 0.05）中异前列腺素浓度最大。
这些结果表明，过氧化SO中脂肪酸组成的变化和/或脂质过氧化产物的存在可能会降低保育猪的ADG和ADFI；但好像对GE、脂质或N消化率，以及肠道通透性没有影响。这些数据表明，脂质过氧化产物的存在可能影响氧化应激的某些标志物。

Influence of feeding thermally peroxidized soybean oil on growth performance, digestibility, gut integrity, and oxidative stress in nursery pigs
The objectives of the current experiments were to evaluate the effect of feeding soybean oil (SO) with different levels of peroxidation on lipid, N, and GE digestibility, gut integrity, oxidative stress, and growth performance in nursery pigs. Treatments consisted diets containing 10% fresh SO (22.5 °C) or thermally processed SO (45 °C for 288 h, 90 °C for 72 h, or 180 °C for 6 h), each with an air infusion of 15 L/min, with postprocessing peroxide values of 7.6, 11.5, 19.1, and 13.4 mEq/kg and p-anisidine values of 1.92, 6.29, 149, and 159, for the 22.5 °C, 45 °C, 90 °C and 180 °C processed SO, respectively. In experiment 1, 64 barrows (7.1 ± 0.9 kg initial BW) were randomly allotted into 2 rooms of 32 pens and individually fed their experimental diets for 21 d, with a fresh fecal sample collected on day 20 for determination of GE and lipid digestibility. In experiment 2, 56 barrows (BW 9.16 ± 1.56 kg) were placed into individual metabolism crates for assessment of GE, lipid, andN digestibility and N retention. Urinary lactulose to mannitol ratio was assessed to evaluate in vivo small intestinal integrity, and urine and plasma were collected to analyze for markers of oxidative stress. Pigs were subsequently euthanized to obtain liver weights and analyze the liver for markers of oxidative stress. In experiment 1, pigs fed the SO thermally processed at 90 °C had reduced ADG (P = 0.01) and ADFI (P = 0.04) compared to pigs fed the other SO treatment groups, with no differences noted among pigs fed the 22.5 °C, 45 °C, and 180 °C SO treatments. No effects of feeding thermally processing SO on dietary GE or lipid digestibility (P > 0.10) were noted in either experiment. In experiment 2, there was no dietary effect of feeding peroxidized SO on the DE:ME ratio, N digestibility, or N retained as a percent of N digested, on the urinary ratio of lactulose to mannitol, on serum, urinary, or liver thiobarbituric acid reactive substances, on plasma protein carbonyls, or on urinary or liver 8-OH-2dG (P > 0.10). In experiment 2, pigs fed the SO thermally processed at 90 °C had the greatest isoprostane concentrations in the serum (P ≤ 0.01) and urine (P ≤ 0.05) compared to pigs fed the unprocessed SO. These results indicate that the change in fatty acid composition and/or the presence of lipid peroxidation products in peroxidized SO may reduce ADG and ADFI in nursery pigs, but appears to have no impact on GE, lipid, or N digestibility, or gut permeability. These data suggest that the presence of lipid peroxidation products may affect certain markers of oxidative stress.

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